Insertion junction: LMJ.SG0182.003841_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.003841
Chromosome:chromosome 2
Location:5226883
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre02.g106050 (1 of 26) IPR029058//IPR029059 - Alpha/Beta hydrolase fold // Alpha/beta hydrolase fold-5 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACGCACCTCCAACA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1218
LEAP-Seq percent confirming:90.1361
LEAP-Seq n confirming:530
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTACCAGAACCCGTAGGC
Suggested primer 2:AAGGTAGGTAGGGCAGCGTT