Insertion junction: LMJ.SG0182.003912_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.003912
Chromosome:chromosome 4
Location:2980908
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g4912 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAACAAAACAGCTTCATAAC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:937
LEAP-Seq percent confirming:94.5806
LEAP-Seq n confirming:733
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGTGTGCGCTTTGAGTT
Suggested primer 2:CTATTTGAGGCACGGGTGTT