Insertion junction: LMJ.SG0182.004861_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.004861
Chromosome:chromosome 12
Location:1616686
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g486350 STPK3,STK3 (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Serine/threonine protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGCTCTGCCCTCCCGAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:787
LEAP-Seq percent confirming:99.5633
LEAP-Seq n confirming:228
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAATCACCATCATCAGC
Suggested primer 2:ACGCACACACACACACACAC