Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.005225 |
Chromosome: | chromosome 16 |
Location: | 4094416 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g669550 | METC,METC1 | Cystathionine beta-lyase; (1 of 1) 4.4.1.8 - Cystathionine beta-lyase / Cystine lyase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGTGTTACCGTCCGCCC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 761 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 32 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGCTACGCCTTCAATTCTT |
Suggested primer 2: | GCAAGAAGTAGACCCGCTTG |