Insertion junction: LMJ.SG0182.005298_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.005298
Chromosome:chromosome 9
Location:6150838
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre09.g405550 TIM21 Mitochondrial inner membrane translocase subunit; (1 of 1) K17796 - mitochondrial import inner membrane translocase subunit TIM21 (TIM21) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGGGAACACGTTACTACA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:682
LEAP-Seq percent confirming:98.8636
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGTTCCTCGTTTCAGTG
Suggested primer 2:TTGCAATAGGGGTACCTTGG