Insertion junction: LMJ.SG0182.005460_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.005460
Chromosome:chromosome 9
Location:5775033
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g10005 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACACACACACGGGTCCGAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1310
LEAP-Seq percent confirming:99.3701
LEAP-Seq n confirming:1262
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTGATAAGGTCGTGGGT
Suggested primer 2:CTTAGAGTCGAGGCCACCTG