Insertion junction: LMJ.SG0182.005623_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.005623
Chromosome:chromosome 10
Location:1471495
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g10588 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTGGTCGTAGCTCGTCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:689
LEAP-Seq percent confirming:96.8153
LEAP-Seq n confirming:152
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCTCAACTCTGTCCCGC
Suggested primer 2:GTGGGTTGGTATGGATGGAG