Insertion junction: LMJ.SG0182.005683_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.005683
Chromosome:chromosome 10
Location:2447372
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g435850 CPLD24 conserved expressed protein, perhaps chloroplast targetted; (1 of 23) IPR008927 - 6-phosphogluconate dehydrogenase C-terminal domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCGGGAAGGGCCAGTACCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1029
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACATACGTCAACTGGAGGGC
Suggested primer 2:GCAGGAGAATGGGTCAAAAA