Insertion junction: LMJ.SG0182.005754_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.005754
Chromosome:chromosome 12
Location:3907951
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g515950 (1 of 35) IPR001763 - Rhodanese-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCATGCGCAGCGCTAGAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:995
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:368
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGCATTTGCTGGTTGGTA
Suggested primer 2:GCGCTGTCACACACAGAAGT