| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.006063 |
| Chromosome: | chromosome 9 |
| Location: | 4052236 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| g9750 | RLS8 | RegA/RlsA-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTACACGAGCACTCGACT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1212 |
| LEAP-Seq percent confirming: | 99.8814 |
| LEAP-Seq n confirming: | 842 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGGTACGTTCGGGTCT |
| Suggested primer 2: | TGGCGATGCATGTAGAGAAG |