Insertion junction: LMJ.SG0182.006104_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.006104
Chromosome:chromosome 17
Location:3509510
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre17.g725150 (1 of 2) K05658 - ATP-binding cassette, subfamily B (MDR/TAP), member 1 (ABCB1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACGACCATGTTGATGCCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1016
LEAP-Seq percent confirming:95.7895
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAATAAGCTGCACCACA
Suggested primer 2:ACTACTTGTGCCGTGCAGTG