Insertion junction: LMJ.SG0182.006595_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.006595
Chromosome:chromosome 6
Location:5359192
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g283550 CRC2,POC6 Proteome of centriole protein 6; (1 of 1) K16466 - centrin-3 (CETN3, CDC31) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACGGGGTCTGTCAGCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:894
LEAP-Seq percent confirming:98.0645
LEAP-Seq n confirming:152
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTTGGCCACTCGCTTTAG
Suggested primer 2:GCTAGCTTCTCCCTGGAGGT