Insertion junction: LMJ.SG0182.006602_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.006602
Chromosome:chromosome 12
Location:4242218
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g519150 (1 of 1) K12169 - Kip1 ubiquitination-promoting complex protein 1 [EC:6.3.2.19] (KPC1, RNF123) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTTGAGCCGAGGGGCCGAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:680
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:191
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCTTGAGGACCTGCATTC
Suggested primer 2:ACGTCGGTTTGTTCTTGTCC