Insertion junction: LMJ.SG0182.006838_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.006838
Chromosome:chromosome 8
Location:3497926
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre08.g375050 FAP86 Flagellar Associated Protein 86 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGAGCGCAGTGTGGAGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:882
LEAP-Seq percent confirming:99.0868
LEAP-Seq n confirming:217
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AATTTACACGCCTGCCAAAC
Suggested primer 2:CACAGCGCCTCAACATAAGA