Insertion junction: LMJ.SG0182.007197_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.007197
Chromosome:chromosome 16
Location:6713694
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g16700 MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCCCGGACACGCTCGAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:839
LEAP-Seq percent confirming:92.6829
LEAP-Seq n confirming:114
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTGCACCATCAACACCA
Suggested primer 2:GGCCTCTGTTGCTACCTCTG