| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.007243 |
| Chromosome: | chromosome 10 |
| Location: | 3315085 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443550 | (1 of 1) 2.7.11.11//2.7.11.12 - cAMP-dependent protein kinase / Protein kinase A // cGMP-dependent protein kinase / PKG II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGAGACCGGGCCCAACA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 97.541 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAGCTGTAGGAGCAGG |
| Suggested primer 2: | CAGCGCTTTATTTCGCCTAC |