Insertion junction: LMJ.SG0182.007378_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.007378
Chromosome:chromosome 6
Location:6372465
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g292150 (1 of 2) PF13499//PF13833 - EF-hand domain pair (EF-hand_7) // EF-hand domain pair (EF-hand_8) 3'UTR
g6768 MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTGCTGATGTCGAAAAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:638
LEAP-Seq percent confirming:97.479
LEAP-Seq n confirming:116
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTTTGGGACTGCACTGTT
Suggested primer 2:AAGGCCGGTATGTTCATCTG