Insertion junction: LMJ.SG0182.007809_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.007809
Chromosome:chromosome 16
Location:5892240
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g677250 TEH5 (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase; Thioesterase-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTAGGTGAAGAGACCCGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1478
LEAP-Seq percent confirming:99.8055
LEAP-Seq n confirming:513
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTTCGGGGATCATTCAGT
Suggested primer 2:CTGTAATCATGGGCCGTCTT