Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.SG0182.007814 |
Chromosome: | chromosome 5 |
Location: | 3414297 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
g5561 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCGCAGCTGGTACTCC |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 726 |
LEAP-Seq percent confirming: | 85.0 |
LEAP-Seq n confirming: | 51 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCTCCAGCAGTAGCACCC |
Suggested primer 2: | AAAATTCTGAAGGGCAGGGT |