| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.008018 |
| Chromosome: | chromosome 10 |
| Location: | 281754 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419800 | PIS1 | (1 of 1) K00999 - CDP-diacylglycerol--inositol 3-phosphatidyltransferase (CDIPT); Phosphatidylinositol synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAGGGCGGCAGGGCAGTG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1248 |
| LEAP-Seq percent confirming: | 99.4937 |
| LEAP-Seq n confirming: | 786 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTAGCGCTTGGCAACTGT |
| Suggested primer 2: | CTTCCCGGATTCTTCACAAA |