| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.008096 |
| Chromosome: | chromosome 2 |
| Location: | 1141551 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081600 | PFH8,PHX3,P4H8 | Prolyl 4-hydroxylase 8; (1 of 14) K00472 - prolyl 4-hydroxylase (E1.14.11.2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGGGCGCCAGCTCGGA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1317 |
| LEAP-Seq percent confirming: | 99.5375 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGAAGTTGTTGTGGGA |
| Suggested primer 2: | AGGATTACGGCACACTGGTC |