Insertion junction: LMJ.SG0182.008144_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.008144
Chromosome:chromosome 6
Location:8026643
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g304150 (1 of 4) PF06985//PF12796 - Heterokaryon incompatibility protein (HET) (HET) // Ankyrin repeats (3 copies) (Ank_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTGCTGTCACTTGTGTCT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:534
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTGCGCCTAGAGTACCGT
Suggested primer 2:CAGGTGACACGGTTTGATTG