Insertion junction: LMJ.SG0182.008215_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.008215
Chromosome:chromosome 10
Location:1246051
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g426700 CYP739A4,CYP28 (1 of 1) 1.14.13.109 - Abieta-7,13-dien-18-ol hydroxylase / CYP720B1; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAAACACGATGGCGCGTGA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:661
LEAP-Seq percent confirming:98.3193
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGTGAAGCTGCTACTGG
Suggested primer 2:GTCGTCAGACAGCACACCAC