Insertion junction: LMJ.SG0182.008578_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.008578
Chromosome:chromosome 9
Location:952618
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre09.g400950 NCT2 (1 of 2) PTHR11662:SF3 - INNER MEMBRANE TRANSPORT PROTEIN RHMT; High-affinity nicotinic acid transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCAAATCATTCATCCTCCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:776
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:140
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGAGCTAACGCTGTGTGT
Suggested primer 2:GACGACGAGACAATGAGCAA