Insertion junction: LMJ.SG0182.008610_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.008610
Chromosome:chromosome 12
Location:1660125
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g486100 CLPP5 active subunit of the chloroplast ClpP complex; (1 of 1) PTHR10381:SF12 - ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 5, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCAACCATCGAGGTGATCG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TACTAATCCTCCGCTCCCCT
Suggested primer 2:GAGATGCTTTCAATGTCGCA