Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.SG0182.008862 |
Chromosome: | chromosome 16 |
Location: | 1400519 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCGCGCCTCTTCACAG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1375 |
LEAP-Seq percent confirming: | 99.9519 |
LEAP-Seq n confirming: | 2080 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCATGTGGAATAGGAATGGG |
Suggested primer 2: | AACCTAACGCGTGTGAATCC |