| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.008918 |
| Chromosome: | chromosome 11 |
| Location: | 1847337 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467900 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAACACACACACGCACA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1390 |
| LEAP-Seq percent confirming: | 99.9321 |
| LEAP-Seq n confirming: | 1471 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATCAAGCCTCTAGCGGGA |
| Suggested primer 2: | GTCCTGCAAAGCTCTTGTCC |