Insertion junction: LMJ.SG0182.009288_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.009288
Chromosome:chromosome 6
Location:1418195
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g259050 (1 of 1) PTHR24365:SF279 - PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTCTGCAGCAAGTGCCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCGTGACTCCATGACT
Suggested primer 2:GTACATGCAGTGTTGTGGGC