| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.009335 |
| Chromosome: | chromosome 14 |
| Location: | 334113 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610050 | (1 of 1) K15180 - negative elongation factor B (COBRA1, NELFB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATCAACGATAATGGAGGA |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1264 |
| LEAP-Seq percent confirming: | 97.9798 |
| LEAP-Seq n confirming: | 1261 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCGCCTCACTATTCCCT |
| Suggested primer 2: | CTTGCTTGAGCCCTTGGTAG |