Insertion junction: LMJ.SG0182.009745_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.009745
Chromosome:chromosome 3
Location:2654283
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre03.g160953 (1 of 1) 3.1.27.1//3.6.3.44 - Ribonuclease T(2) / Ribonuclease T2 // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCAGCGAGTGCACGGACGA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:78.3333
LEAP-Seq n confirming:141
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCCAAACTAAACCAAA
Suggested primer 2:CGGTGTGTGTGTGTGTGTGT