Insertion junction: LMJ.SG0182.009777_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.009777
Chromosome:chromosome 2
Location:1387721
Confidence (%):75
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACTCGACATCTGTCGATTA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:721
LEAP-Seq percent confirming:99.3377
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCATTACGGGGGTACAGAGC
Suggested primer 2:TGCCATGCAACATGATTTTT