Insertion junction: LMJ.SG0182.009844_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.009844
Chromosome:chromosome 12
Location:2839293
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g502350 HEL50 (1 of 1) K10899 - ATP-dependent DNA helicase Q1 [EC:3.6.4.12] (RECQL); DEAD/DEAH box DNA/RNA helicase, related to RECQ1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCTGGAGCGACCAAATGGAT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:562
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGTGCAGTGTAGGGAACG
Suggested primer 2:CTAGAAGCGCGAAACAAACC