Insertion junction: LMJ.SG0182.010231_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.010231
Chromosome:chromosome 6
Location:2015929
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre06.g264250 FAO6 FAD-dependent oxidoreductase; (1 of 3) 5.5.1.19 - Lycopene beta-cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCATGCGCGGCATGCAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:929
LEAP-Seq percent confirming:80.5263
LEAP-Seq n confirming:306
LEAP-Seq n nonconfirming:74
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCACGTGTCATTGGTGTGTG
Suggested primer 2:CTGACTCCAAGCCCTCACTC