Insertion junction: LMJ.SG0182.010429_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.010429
Chromosome:chromosome 7
Location:4226059
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre07.g342000 (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGGGCGGGTGGTTGCGTT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:633
LEAP-Seq percent confirming:98.8372
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTCCACCATCCGAGTTG
Suggested primer 2:GCACACACACCAATCACACA