Insertion junction: LMJ.SG0182.010495_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.010495
Chromosome:chromosome 10
Location:3700627
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g446100 TRX6,TRXY,TRXY1 Thioredoxin y, chloroplastic; (1 of 1) PTHR10438:SF280 - THIOREDOXIN Y1, CHLOROPLASTIC-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTCTTGAGGGGTACAGGGA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1093
LEAP-Seq percent confirming:99.6885
LEAP-Seq n confirming:320
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGCTTGCCCTTGATACTG
Suggested primer 2:TGAGTGTGGAGTGGAACCAA