Insertion junction: LMJ.SG0182.010810_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.010810
Chromosome:chromosome 12
Location:1680523
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g485900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATTGTCCAGCCTCCACGCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:809
LEAP-Seq percent confirming:99.6774
LEAP-Seq n confirming:309
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACATGGCAAGGTTGATG
Suggested primer 2:TGCTTGTCAGCAACAAGGAC