Insertion junction: LMJ.SG0182.011008_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.011008
Chromosome:chromosome 2
Location:3425694
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g1884 VSR1 Vacuolar Sorting Receptor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCACCGCACCGCCTGCC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:768
LEAP-Seq percent confirming:99.7135
LEAP-Seq n confirming:348
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCACCATGCACACAACA
Suggested primer 2:ACGGGCAAACTACAAACGAC