Insertion cassette: | pMJ013b |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.SG0182.011065 |
Chromosome: | chromosome 12 |
Location: | 1422943 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g487950 | (1 of 2) IPR000742//IPR017853 - EGF-like domain // Glycoside hydrolase superfamily | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGTGTCATTGCTGACGAT |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 886 |
LEAP-Seq percent confirming: | 99.3827 |
LEAP-Seq n confirming: | 161 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGTACCCATGTCACACACA |
Suggested primer 2: | ATACATGCCGGCACCTCTAC |