Insertion junction: LMJ.SG0182.011183_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.011183
Chromosome:chromosome 10
Location:950902
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre10.g424700 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTGCATCCACCGCATGAAG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1273
LEAP-Seq percent confirming:96.5021
LEAP-Seq n confirming:469
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGTATGTGGACATTGCCG
Suggested primer 2:GAAAAACGAAATCCGCGTAA