Insertion junction: LMJ.SG0182.011261_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.011261
Chromosome:chromosome 14
Location:2388025
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre14.g624350 VTE6 Tocopherol O-methyltransferase; (1 of 1) K18534 - MPBQ/MSBQ methyltransferase (K18534) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAATCTGAAGCCTATGAGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:99.6463
LEAP-Seq n confirming:1972
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AAACTCCACAAACGACAGGG
Suggested primer 2:GAGTTGGGCAGGGAGTATGA