| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.011485 |
| Chromosome: | chromosome 4 |
| Location: | 1996208 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218750 | THB4 | Truncated hemoglobin; (1 of 1) IPR012292 - Globin/Protoglobin | MULTIPLE_SPLICE_VARIANTS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTCAGAATTGCATACAAG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1275 |
| LEAP-Seq percent confirming: | 96.8706 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACTCCGATACATTCGTT |
| Suggested primer 2: | GGAGTGGACATGGTAACGCT |