Insertion junction: LMJ.SG0182.011505_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.011505
Chromosome:chromosome 3
Location:8727377
Confidence (%):75
Locus systematic id Locus common name Defline Feature
g4408 PDE21 3\',5\'-cyclic-nucleotide phosphodiesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAATGCTGTCGCAGTGGAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1455
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:717
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTTGTCAGGAACGGGAA
Suggested primer 2:CATCACCACTGCAAAACCAC