| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.011872 |
| Chromosome: | chromosome 12 |
| Location: | 3100352 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500100 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGTGTACCAGCGACAG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1121 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 520 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGAGAGCTCTTGATCTGA |
| Suggested primer 2: | CGTTTGATTGAGTTCGCTGA |