| Insertion cassette: | pMJ013b |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.SG0182.012025 |
| Chromosome: | chromosome 13 |
| Location: | 2562758 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580900 | (1 of 1) K07178 - RIO kinase 1 (RIOK1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCATGCAGCCTGCTCCC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCTGCATGGTTCTGTGC |
| Suggested primer 2: | AACCTCTTTCTCCCTCGCTC |