| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.012376 |
| Chromosome: | chromosome 12 |
| Location: | 4454882 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521400 | (1 of 1) 6.3.5.11 - Cobyrinate a,c-diamide synthase (glutamine-hydrolyzing) / Cobyrinic acid a,c-diamide synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTATCAGCACCTCAAACCAC |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1392 |
| LEAP-Seq percent confirming: | 99.9648 |
| LEAP-Seq n confirming: | 2842 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGGCACATAAGAGGCAC |
| Suggested primer 2: | CATCATCATCCTCACATCGC |