Insertion junction: LMJ.SG0182.012713_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.012713
Chromosome:chromosome 1
Location:6764091
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre01.g048650 (1 of 2) PTHR24347//PTHR24347:SF159 - SERINE/THREONINE-PROTEIN KINASE // SERINE/THREONINE-PROTEIN KINASE STK11 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTATCTAGGTCGGGACAAGT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:789
LEAP-Seq percent confirming:99.4475
LEAP-Seq n confirming:180
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGGACGAGCTTCTTGCAT
Suggested primer 2:GGGATGGTGAAGGTCGTAGA