Insertion junction: LMJ.SG0182.013299_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.013299
Chromosome:chromosome 17
Location:1621692
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre17.g708000 FAP260 Membrane-associated Flagellar Associated Protein 260; (1 of 11) PF13426 - PAS domain (PAS_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAGTCTTGCAACCATTTGA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1327
LEAP-Seq percent confirming:99.8735
LEAP-Seq n confirming:2368
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACTCGTGCTGTGCTTTG
Suggested primer 2:GACTTCTTCTTGTGCAGGGC