Insertion junction: LMJ.SG0182.013441_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.013441
Chromosome:chromosome 3
Location:4859756
Confidence (%):75
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCTTCCCGCCCGCTGGCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:846
LEAP-Seq percent confirming:75.7238
LEAP-Seq n confirming:340
LEAP-Seq n nonconfirming:109
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGTACTGCTCCCTCAGC
Suggested primer 2:ACGGGTCTGTGAGTACTGGG