Insertion junction: LMJ.SG0182.013561_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:+
Strain:LMJ.SG0182.013561
Chromosome:chromosome 7
Location:3588462
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre07.g336650 MLH3 DNA mismatch repair protein; (1 of 1) IPR003594//IPR020568 - Histidine kinase-like ATPase, C-terminal domain // Ribosomal protein S5 domain 2-type fold MULTIPLE_SPLICE_VARIANTS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGCCGTAGCGCGTGCGG
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:1353
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:202
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCAGCGATACAGAGCAAG
Suggested primer 2:CAACTACACCACTAGGCGCA