Insertion junction: LMJ.SG0182.013561_2

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.013561
Chromosome:chromosome 16
Location:3215911
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre16.g666500 BBS8 (1 of 1) K16781 - tetratricopeptide repeat protein 8 (TTC8, BBS8); Bardet Biedl Syndrome-8 associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGGCCGGGAGATGCCGCAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:901
LEAP-Seq percent confirming:99.7416
LEAP-Seq n confirming:386
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTCGAAGCTGACTTGGAA
Suggested primer 2:CTCCCTGACTCTCTTGCCAC